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. 2006 Feb 3;4:1. doi: 10.1186/1741-7007-4-1

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Copyright © 2006 Weinkove et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of starvation on DAF-16 localisation and activity. (A-D) Images of live L1 larvae containing the muIs71 transgene expressing DAF-16a::GFP in a daf-16(mu86) background. Subcellular localisation of DAF-16a::GFP is clearest in the neurons of the nerve ring indicated by arrows. (A) fed larva, cytoplasmic and nuclear, (B) starved day 1, nuclear (C) starved day 2, cytoplasmic (with a characteristic "honeycomb" appearance) (D) age-1(hx546) starved day 2, nuclear (E) Quantification of the effect of prolonged starvation on DAF-16a::GFP localisation in wild type and age-1(hx546) L1 larvae. n= number of worms imaged. Number of independent experiments in brackets.