Table 1.

The nucleotide sequences and fluorescent labels of the primers for RT-PCR of LHB/CGB and reference genes.

Gene	Primer
		F/R	Sequence with Fluorescent Dye*	Tm (ºC)	Product size
CGB	}	F	5′-GACCCCACCATAGGCAGAG-3′	61	541bp
CGB5
CGB7		R	5′-GGTAGTTGCACACCACCTGA-3′ FAM	59.4
CGB8
CGB1	}	F	5′-GGAGGGAGGAAGGGGAACT-3′	61	207bp
CGB2
CGB1		RG	5′-GCAACAGCAGCAGCCTCTTT-3′ FAM	59.4
CGB2		RG	5′-CAACAGCAGCAGCCCCTTT-3′ FAM	58.8	206bp
GAPDH		FML	5′-CCATGGAGAAGGCTGGGG-3′	60.5	196bp
		RML	5′-CCAAGTTGTCATGGATGACC-3′ HEX	57.3
RPII		F	5′-CTTCACGGTGCTGGGCATT-3′	58.8	239bp
		RR	5′-GTGCGGCTGCTTCCATAA-3′ TET	56
LHB**		FG	5′-GCTACTGCCCCACCATGATG-3′	61.4	94bp
		R1G	5′-ATGGACTCGAAGCGCACATC-3′ FAM	59.4
		R2	5′-AGAGCCACAGGGAAGGAGAC-3′ FAM	61.3	151bp
		R3	5′-AGCTGAGAGCCACAGGGAAG-3′ FAM	61.4	156bp

Tm, melting temperature; F, forward primer; R, reverse primer; a primer originally published by

^GGiovangrandi et al., 2001

^MLMiller-Lindholm et al., 1997

^RRadonic et al.

^*6-FAM, TET and HEX emit fluorescent signals at 532nm, 543nm, 557nm of wavelengths, respectively.

^**For LHB, one forward primer was combined with different reverse primers.