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. Author manuscript; available in PMC: 2006 Mar 20.
Published in final edited form as: J Biol Chem. 2004 Oct 20;279(53):55147–55152. doi: 10.1074/jbc.M410826200

FIG. 4.

FIG. 4

Reversible isoform-selective inhibition of GLUT4 activity by z-His-Phe-Phe-O-Et.A, [3H]2-deoxyglucose uptake (30 min at 22 °C) was measured 3 days following the injection of mRNA for each GLUT isoform into stage 5 Xenopus oocytes. The zHFFe peptide (dis-solved in dimethylformamide) was added 6 min prior to initiating the uptake assay. An equivalent volume of vehicle was added to the zero peptide control. Results represent the mean ± S.E. of three independent experiments. Asterisk (*) indicates p < 0.05. B, oocytes (15-20/group) were incubated for 60 s with 200 μM of z-His-Phe-Phe-O-Et (Peptide + Wash) or Bath's saline (Control). Oocytes were then either washed with peptide-free Barth's saline (Wash) or incubated in the continued presence of peptide (Peptide) during the 2-deoxyglucose up-take assay. Results represent the mean ± S.E.; asterisk (*) indicates p < 0.01 versus control.