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Species-specific DNA analysis of E. dispar and E. histolytica isolates. (A and B) Locus 1-2. Amplification products were generated with primers Dsp1 and -2 (Ed) and primers Hsp1 and -2 (Eh) at an annealing temperature of 50°C. (C and D) Locus 5-6. Amplification products were generated using primers Dsp5 and -6 (Ed) at an annealing temperature of 52°C and primers Hsp5 and -6 (Eh) at an annealing temperature of 48°C.
