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. 2002 Apr;40(4):1303–1310. doi: 10.1128/JCM.40.4.1303-1310.2002

FIG. 3.

FIG. 3.

DNA hybridization of various mycobacterial species shows that genes 251, 253, and 255 are present only in M. avium subsp. paratuberculosis. The 1% agarose gel in panel A containing PstI-restricted DNA fragments was transferred to a nylon membrane and sequentially probed with genes 255 (B), 253 (C), and 251 (D). The nylon filter was stripped between hybridizations. An M. avium subsp. paratuberculosis DNA fragment greater than 9.4 kb was detected in each hybridization. λ-HindIII size standards are indicated on the left. Lanes: 1, M. fortuitum; 2, M. bovis BCG; 3, M. intracellulare; 4, M. avium subsp. paratuberculosis K-10; 5, M. avium subsp. avium TMC801.