Table 1. Kinetic parameters for the aminoacylation of hs mt tRNALeu(UUR) constructs.
Km (µM) | kcat (s–1) | kcat / Km (relative) | |
---|---|---|---|
WT | 2.3 ± 0.5 | 0.020 ± 0.002 | 1 |
U3271C | 9.7 ± 0.9 | 0.024 ± 0.001 | 0.3 |
A3261G/U3271C | 2.3 ± 0.4 | 0.047 ± 0.004 | 2.3 |
Km (µM) | kcat (s–1) | kcat / Km (relative) | |
---|---|---|---|
WT | 2.3 ± 0.5 | 0.020 ± 0.002 | 1 |
U3271C | 9.7 ± 0.9 | 0.024 ± 0.001 | 0.3 |
A3261G/U3271C | 2.3 ± 0.4 | 0.047 ± 0.004 | 2.3 |