Table 2. Summary of all hybridization data, comparing expected and observed hybridization signals for each test species in regions A and B of the 16S rRNA gene.
| Test species | P.veronii | B.pumillus | P.aeruginosa | P.alcaligenes | P.fluorescens | P.putida | P.syringae | Total | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Region | A | B | A | B | A | B | A | B | A | B | A | B | A | B | A + B |
| Total expected | 4 | 6 | (–) | 8 | 5 | 26 | 5 | 12 | 4 | 7 | 4 | 5 | 4 | (–) | 90 |
| Positive/expected | 1 | 0 | (–) | 5 | 3 | 17 | 2 | 4 | 4 | 4 | 3 | 3 | 3 | (–) | 49 |
| Negative/expected | 3 | 6 | (–) | 3 | 2 | 9 | 3 | 8 | 0 | 3 | 1 | 2 | 1 | (–) | 41 |
| Positive/unexpected | 3 | 5 | (–) | 8 | 7 | 13 | 3 | 11 | 4 | 10 | 3 | 17 | 4 | (–) | 88 |
Total expected signals are based on the existence of perfect sequence matches within the PCR targets. Positive/expected signals represent the detection of hybridization for probes with a perfect match within the target sequence. Negative/expected signals represent the lack of detectable hybridization for probes possessing a perfect match within the target sequence. Positive/unexpected signals represent the detection of hybridization signals for probes lacking a perfect match within the target sequence (mismatched hybridization). (–), untested.