Figure 2.

Effect of C.Esp1396I on the expression of esp1396IM gene. Bacterial strain harboring both plasmid pEspM::Lac (contains esp1396IM’::’lacZ gene fusion) and plasmid pZS-C (contains functionally active gene for regulatory protein under the control of P_lac/ara-1) was cultivated either with or without of P_lac/ara-1 inducers arabinose and IPTG. Open columns indicate β-Gal activity expressed in Miller units in the presence of both inducers (C.Esp1396I synthesis activated) measured at the time points indicated on the bottom of the scheme; shaded columns indicate β-Gal activity obtained at the same time points in the absence of both inducers (C.Esp1396I synthesis repressed). Numbers at the bottom of each column represent the total number of probes measured in three independent experiments for each time point; thin line at the top of each column indicates standard deviation.