Table 1. Effect of C.Esp1396I on expression of esp1396IC and esp1396IR gene fusions.
| Plasmid with gene fusion investigated | Auxiliary plasmid | Localization of regulatory genea | β-Gal activityb |
|---|---|---|---|
| pEspC::Lac (esp1396IC’::’lacZ) | pBR322 | – | 6.3 ± 0.39 (9) |
| pEspC::Lac (esp1396IC’::’lacZ) | pBR-C | In trans | 301 ± 16.2 (8) |
| pEspCR::Lac (esp1396IR’::’lacZ) | pBR322 | In cis | 63.6 ± 7.0 (9) |
| pEsp+4CR::Lac (esp1396IR’::’lacZ) | pBR322 | – | 0.46 ± 0.07 (9) |
| pEsp+4CR::Lac (esp1396IR’::’lacZ) | pBR-C | In trans | 2.41 ± 0.21 (9) |
| pEsp+12CR::Lac (esp1396IR’::’lacZ) | pBR322 | – | 4.6 ± 0.56 (9) |
| pEsp+12CR::Lac (esp1396IR’::’lacZ) | pBR-C | In trans | 82 ± 8.6 (9) |
aPlasmid pEspCR::Lac contains an active regulatory gene; –, regulatory gene in these cells is absent.
bValues indicate mean activities of hybrid β-Gal in Miller units; ±, the standard error; numbers in parentheses represent the total number of probes measured in three independent experiments.