Figure 6.

hMcm8 protein does not immunoprecipitate with the P1/Mcm2–7 protein complexes. The extracts of exponentially growing HeLa cells were prepared as described. An aliquot of one-tenth of the extract was loaded as input. The extracts were immunoprecipitated with anti-hMcm3 or anti-hMcm4 antibodies or with rabbit immunoglobin as control (control). (A) Immunoprecipitation using anti-hMcm3 antibody. The blots were revealed with anti-hMcm3, anti-hMcm5 or anti-hMcm8 antibodies. (B) Immunoprecipitation using anti-hMcm4 antibody. The blots were revealed with anti-hMcm4, anti-hMcm6, anti-hMcm2 or anti-hMcm-8 antibodies.