Table 2. Mutational properties of 8-OH-Gua in mammalian cells.

Sequence	ds/ss	Host	MF (%)	Mutations detected				Reference
(5′→3′)a				G→T	G→A	G→C	Others
GG*C	ds	NIH3T3	∼1.4b	8	3	1	2c	19
GG*C	ds	NIH3T3	∼1.7b	22	8	1	11d	20
CG*G	ds	NIH3T3	∼0.6b	14	1	0	0	20
TG*G	ds	NIH3T3	∼1.0b	35	0e	0e	5f	20
CG*T	ss	COS-7	4.2	15g	0	1	2h	21
CG*G	ss	COS-7	4.0	12	0	0	0	22
GG*C	ss	COS-7	4.0	12	0	0	1i	22
TG*G	ss	COS-7	5.2	10	1	2	0	23
GG*C	ss	COS-7	6.8	22	3	0	3j	23

^aG* represents 8-OH-Gua.

^bMF is calculated on the focus formation relative to that of activated c-Ha-ras genes.

^cTwo mutants contained a mutation at a flanking site.

^dEleven mutants contained a mutation at a flanking site.

^eMutations at this position do not activate the c-Ha-ras gene. Mutants may fail to be selected due to lack of focus-formation.

^fFive mutants contained a mutation at a flanking site.

^gOne mutant contained a double mutation (CG*T→ATT).

^hTwo mutants contained a mutation at a flanking site.

ⁱThe mutant contained a deletion.

^jThe mutants contained a mutation at a flanking site.