Figure 5.

Yeast two-hybrid binding assay. The binding of the different ZmORC subunits is shown when expressed as AD- or BD-expressors. + and – show yeast growth in a positive and a negative control, respectively. The binding of ZmORC1 to combinations of multiple co-expressed subunits is shown in the last row. The figures in the brackets correspond to the ZmORCs expressed simultaneously. In each plate, four independent interactions are shown in parallel, except in the last row where eight independent reactions are shown.