Figure 3.

Penetratin allows the transfer of DNA oligonucleotides to a hydrophobic environment. Various amounts of Penetratin (Pen) or W6F mutant peptide (PenWF) were added to ³²P-labeled dsDNA at 16 pM, conditioned in Tris 10 mM pH 7.4. PS was conditioned in chloroform. After mixing and decanting the phases, the radioactivity retrieved from both phases was counted and reported to that of the input (relative c.p.m. of 100). Only the data corresponding to the water phase are shown. Bars indicate the standard variation of at least three independent experiments.