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. 2003 Jan 15;31(2):556–561. doi: 10.1093/nar/gkg160

Figure 5.

Figure 5

Internalized DNA oligonucleotides reach the nucleus of COS7 cells at 4°C. Penetratin (100 µM) and/or FITC-labeled dsDNA oligonucleotides (0.2 µM) were preincubated in DMEM medium and added to COS7 cells for 2 h at 4°C. Cells were then processed for visualization under confocal microscopy. (A) Intracellular fluorescence detected after incubation with Penetratin and FITC dsDNA, at 4°C. (B) Intracellular fluorescence detected after incubation at 4°C with FITC dsDNA oligonucleotides alone.