Table 1. Telomerase activity in human hepatocytes and hepatoma cell lines.
| Cells | TRAP-ELISAa | RQ-TRAP | TRAPezeb |
|---|---|---|---|
| Human hepatocytes | |||
| Fetal-04/00 | 0.3 ± 0.16 (n = 4) | 0.5 ± 0.14 (n = 4) | + (n = 2) |
| Fetal-10/01 | 0.9 ± 0.53 (n = 4) | 1.1 ± 0.30 (n = 4) | + (n = 2) |
| Pediatric-11/01 | 0.2 ± 0.15 (n = 2) | 1.2 ± 0.11 (n = 4) | + (n = 2) |
| Pediatric-27/01 | 0.0 ± 0.01 (n = 2) | 1.8 ± 0.15 (n = 4) | + (n = 2) |
| Pediatric-13/01 | 0.0 ± 0.00 (n = 2) | 1.7 ± 0.15 (n = 2) | + (n = 2) |
| Pediatric-26/02 | 0.0 ± 0.00 (n = 4) | 0.4 ± 0.10 (n = 2) | + (n = 2) |
| Adult-23/00 | 1.0 ± 0.85 (n = 2) | 1.0 ± 0.38 (n = 4) | + (n = 2) |
| Adult-09/01 | 0.2 ± 0.10 (n = 2) | 0.8 ± 0.09 (n = 4) | + (n = 2) |
| Hepatoma cell lines | |||
| Hep G2 | 51.2 ± 3.40 (n = 4) | 57.4 ± 3.63 (n = 8) | ND |
| HLE | 35.5 ± 8.11 (n = 4) | 22.7 ± 1.02 (n = 6) | ND |
| HLF | 40.3 ± 7.57 (n = 4) | 36.8 ± 2.81 (n = 6) | ND |
| HuH 7 | 44.3 ± 6.75 (n = 4) | 37.3 ± 0.43 (n = 6) | ND |
All values are expressed as means ± SEM of the indicated number of measurements. Telomerase activity is tabulated as RTA per 1000 cells. ND, not determined.
aThe quantitative TeloTAGGG Telomerase PCR ELISAPLUS was employed to quantitate telomerase activity.
bLow RQ-TRAP results were confirmed with the qualitative TRAPeze ELISA Telomerase Detection Kit.