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Comparative maps of the baculovirus polyhedrin locus. (A) The polyhedrin locus and surrounding genes on the wild type AcMNPV (accession no. L22858) genome. (B) The intermediate viral derivative BacPak6 showing the position of Bsu36I sites introduced to allow genome linearisation prior to transfection (10). Note, Bsu36I sites present in the β-galactosidase gene are not shown. (C) The AcMNPV bacmid used for insertional mutagenesis of ORF1629.
