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Melting behavior of conventional and shared-stem molecular beacons with a 19-base probe and a five-base stem. (A) Melting curves for conventional (green) and shared-stem (red) molecular beacons in the presence of wild-type (solid line) and mutant (dashed line) target. (B) The difference in the fraction of conventional (green) or shared-stem (red) molecular beacons bound to wild-type and mutant targets.
