Table 2. Changes in enthalpy and entropy of conventional and shared-stem molecular beacons in the presence of target.

Target	Probe length	Conventional molecular beacons			Shared-stem molecular beacons
		Stem length	ΔH (kJ/mol)	ΔS (J/mol·K)	Stem length	ΔH (kJ/mol)	ΔS (J/mol·K)
WT	19	4	823 ± 168	2281 ± 489	4	862 ± 116	2383 ± 336
A	19	4	577 ± 62	1595 ± 184	4	708 ± 36	1967 ± 106
B	19	4	527 ± 27	1471 ± 79	4	586 ± 54	1628 ± 161
C	19	4	472 ± 23	1336 ± 70	4	478 ± 49	1340 ± 148
D	19	4	480 ± 38	1352 ± 115	4	521 ± 87	1461 ± 262
WT	19	5	649 ± 28	1784 ± 83	5	690 ± 16	1887 ± 46
A	19	5	418 ± 23	1133 ± 70	5	446 ± 20	1205 ± 59
B	19	5	385 ± 24	1055 ± 73	5	391 ± 24	1057 ± 72
C	19	5	324 ± 17	901 ± 54	5	369 ± 57	1025 ± 175
D	19	5	291 ± 25	790 ± 76	5	319 ± 28	861 ± 85
WT	19	6	467 ± 16	1265 ± 48	7	413 ± 10	1096 ± 28
A	19	6	404 ± 25	1105 ± 76	7	370 ± 13	998 ± 38
B	19	6	380 ± 26	1055 ± 79	7	351 ± 39	956 ± 117
C	19	6	367 ± 19	1058 ± 61	7	260 ± 30	707 ± 93
D	19	6	373 ± 29	1065 ± 91	7	245 ± 25	653 ± 79