Figure 3.

The cleavage reactions of TAR RNA with Pb²⁺ (pH 7.2, 15 min, 37°C), Mg²⁺ (pH 8.5, 15 h, 37°C) and Ca²⁺ (pH 8.5, 15 h, 37°C) ions. The concentrations of cations (mM) are indicated above the lanes. L and T indicate formamide ladder and partial hydrolysis by RNase T1, respectively. The relative intensities of cleavages induced at the bulge region by 0.5 mM Pb²⁺, 2 mM Mg²⁺ and 2 mM Ca²⁺ are shown below.