Table 1. Characterization of sample cDNA fragment libraries.
| Tissue sourcea | mRNA (µg) | Clones (n) | Percent 5′ regionb (n) |
|---|---|---|---|
| Proximal limb buds (E12.5) | 3.0 | 3.8 × 105 | 84 |
| Ribs (E16.5) | 4.6 | 4.8 × 105 | 91 |
| SaOS-2 cell line | 4.0 | 4.0 × 105 | 89 |
| Limb buds (E14.5) | 3.0 | 5.9 × 105 | 64 |
| UMR-106 cell line | 4.3 | 7.3 × 105 | 85 |
aTissues are dissected from mouse embryos at the indicated stage.
bPercentage of inserts with homology to known sequences corresponding to the 5′ region of the cDNA, determined from random sequencing of 40–48 clones.