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. 2002 Oct 1;30(19):4166–4175. doi: 10.1093/nar/gkf548

Figure 4.

Figure 4

Reconstitution of H.volcanii SRP. Haloferax volcanii SRP19 and SRP54 were combined with SRP RNA either separately or together and the resulting complexes were examined by sucrose density gradient centrifugation. The levels of each component in the fractions collected from each gradient were determined densitometrically following electrophoresis and are presented as units. (A) Gradients prepared in 1 M KCl. (B) Gradients prepared in low salt. In both (A) and (B), the upper panels present the level of SRP19 in fractions collected from gradients containing SRP19 alone (filled circles), as well as the levels of SRP RNA (open circles) and SRP19 (filled squares) in fractions collected from gradients to which both components were added together. The middle panels in (A) and (B) present the levels of SRP54 in fractions collected from gradients containing SRP54 alone (filled circles), as well as the levels of SRP RNA (open circles) and SRP54 (filled squares) in fractions collected from gradients to which both components were added together. The lower panels in (A) and (B) present the levels of SRP RNA (filled circles), SRP19 (filled squares) and SRP54 (open circles) in fractions collected from high and low salt containing gradients containing all the components, respectively. In the lower panel of (B), the level of SRP RNA (open squares) in fractions collected from a low salt gradient to which SRP RNA alone was added is also shown.