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. 2002 Oct 1;30(19):4199–4207. doi: 10.1093/nar/gkf551

Figure 1.

Figure 1

Schematic drawing of the HTA1–AKY2 intergenic region. (A) Positions of 5′ ends of primers used in PCR amplification are indicated by arrows and numbers (adenine residue of the AUG translational start triplet as +1). (TA)6, TATA element. Asterisks denote transcriptional initiation sites. (B) Promoter truncations. (C) TATA mutations made in pH4. TΔ12, complete deletion of the TATA element; Tmut, the central part of the element has been interrupted by site-directed mutagenesis; Tcons, exchange of the (TA)6 element for a canonic TATAAA box. In H4Δ21, 21 bp have been deleted 5′ adjacent to the TATA-like element; in H4Δ33, 33 bp including the (TA)6 element and the 5′ kink have been deleted. Right, β-galactosidase activities (nmol/mg·min) of the respective mutant promoter/lacZ fusion constructs ligated to the low copy expression vector, pYLZ7. Numbers in brackets denote percentage of activity obtained with construct HN.