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. 2002 Oct 1;30(19):4127–4137. doi: 10.1093/nar/gkf553

Figure 1.

Figure 1

Figure 1

Figure 1

1,10-phe can efficiently inhibit the second step of mRNA splicing. (A) The molecular structure of 1,10-phe and 1,7-phe. Zinc is schematically added at its potential binding site. (B) A radiolabeled transcript of Adeno was incubated in an in vitro splicing reaction. The indicated concentration of the zinc chelating agent 1,10-phe or the non-chelating zinc agent 1,7-phe was added to each reaction and the reactions were incubated for 90 min at 30°C. RNA was extracted and separated by PAGE in 8% denaturing gel. RNA intermediates and products are schematically presented on the left; exons are represented by boxes and introns by lines. (C) The inhibition of the first and second step of splicing products—3′ exon-lariat (squares) and mRNA (circles)—as a function of the concentration of 1,10-phe in three different experiments. Minimum and maximum levels of the first and second steps of splicing products were normalized to a relative intensity of zero and 100, respectively.