Figure 3.
The snRNAs are not degraded by the addition of 1,10-phe. Nuclear extract was incubated in in vitro splicing conditions for 60 min at 30°C with the indicated concentration of 1,10-phe and CuSO4. RNA was extracted, separated by electrophoresis in a 10% polyacrylamide gel, and transferred to a nylon membrane. Following hybridization with U1, U2, U4, U5 and U6 complementary labeled antisense riboprobes, the membrane was washed and exposed to X-ray film. The snRNAs were identified according to size and are marked on the left.