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NC and BC can efficiently inhibit the second step of mRNA splicing. (A) The molecular structure of NC and BC. Copper is schematically added at its potential binding site. (B) A radiolabeled transcript of Adeno was incubated in an in vitro splicing reaction with the indicated concentration of NC and BC. RNA was extracted and separated in 8% denaturing gel. RNA intermediates and products are schematically presented on the left.
