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Comparison of Cdc13 DBD 1H-15N HSQC spectra. (A) 1H-15N HSQC spectrum of 400 µM 451–694 DBD at 600 MHz, 20°C. The sample was prepared in 50 mM potassium phosphate, pH 7.0, 50 mM NaCl, 0.02% NaN3, 2 mM DTT-d10 and 10% D2O. (B) 1H-15N HSQC spectrum of 700 µM 497–694 DBD at 600 MHz, 25°C. The sample was prepared in 50 mM imidazole-d4, pH 7.0, 150 mM NaCl, 100 mM Na2SO4, 0.02% NaN3, 2 mM DTT-d10 and 10% D2O.
