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MALDI-TOF analyses of DNA cleavage reaction by PI-PabI (A–D) and PI-PabII (E–H). Aliquots of 50 pmol of synthetic DNA were incubated with 1 µg of PI-PabI or 10 µg of PI-PabII in the corresponding optimal buffers at 70°C, for 2 min [(A) and (E)], 5 min [(B) and (F)], 15 min [(C) and (G)] and 60 min [(D) and (H)]. Digested DNA (1 pmol) was analysed as described.
