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Denaturing PAGE analysis of pentamer DNA with mixed protecting groups illustrating the difference in reactivity between the Dma protecting group of dA and the ultra-mild deprotection groups for the conditions described (see also text). Slower moving bands are Dma-protected DNA. Conditions: lane 1, AMA, 8 h; lane 2, K2CO3/MeOH, 12 h; lane 3, K2CO3/MeOH, 12 h, then AMA, 8 h; lane 4, NH4OH, 55°C, 4 h; lane 5, NH4OH, 55°C, 8 h; lane 6, native ssDNA marker.
