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Gel analysis of S1 endonuclease cleavage of the circular product [cyclic (dCCCTAA)9] and the linear precursor (PAGE, 20%). Circularity is confirmed by the lack of banding between the bands corresponding to the circular and linear DNA (see text for details). Lane 1, circular DNA, no nuclease S1; lane 2, circular DNA, S1 reaction; lane 3, linear DNA, no S1; lane 4, linear DNA, S1 reaction.
