FIG. 1.

Identification of the N termini of the VP3 and VP4 proteins. (A) Schematic representation of the VP4-3Δ0 construct expressed in E. coli. The numbers indicate the amino acid position in the polyprotein, and the horizontal arrows indicate the N termini of VP3 and VP4. (B) SDS-PAGE analysis of the bacterial extracts following (+) or not following (−) induction with 1 mM IPTG (isopropyl-β-d-thiogalactopyranoside). The two induced bands were excised and submitted to seven Edman degradation cycles.