FIG. 2.
Partially double-stranded nucleic acid substrates used in the enzyme assays. (A) An RNA substrate. The template strand (05) and the release strand (06) were chemically synthesized in both ssRNA and ssDNA and annealed to form a dsRNA or a dsDNA substrate. The 5′ end of the release strand was first labeled with [γ-32P]ATP before annealing. The numbers above the substrate represent the position of the nucleotide in the HCV genome. (B) The nucleotide sequence and the secondary structure of the 3′ end of HCV genome. The portion of the sequence that was used as the substrate is numbered.