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The time course of the unwinding reaction. The unwinding reaction was performed with dsRNA (•) or dsDNA (○). The reaction was started by adding 20 nM HCV NS3 helicase and stopped by adding a termination buffer. The reaction product was resolved on a native 15% polyacrylamide gel immediately after incubation for the indicated time and then quantified using a phosphorimager. The solid lines represent nonlinear least-squares fits of the data to the exponential equation.
