FIG. 6.
Impaired virus transmission by Mm-CD209L2. Relative virus capture and transmission by CD209 family molecules was assayed with HIV-Luc pseudotyped with HIV-1 ADA Env (A) or SIVMAC1A11 Env (B). THP-1, THP-1/Hs-CD209, THP-1/Mm-CD209, and THP-1/Mm-CD209L2 donor cells were preincubated with the MAbs (10 μg/ml) for 30 min at 37°C, after which HIV-Luc pseudotypes were added and the mixture was incubated for an additional 3 h at 37°C. The cells were then washed and cocultured with Hut/CCR5 target cells in the presence of Polybrene. HIV-1 infection was determined after 2 days by measuring the luciferase activity. Treatment with MAb 526 was used to evaluate the necessity of CD209 molecules for transmission, and mouse IgG (mIgG) was used as a nonspecific antibody control. Each set of data represents the mean of three separate wells of infected cells. The results of one experiment representative of three are shown. cps, counts per second.