Figure 2.

Cleavage with mAMSA and a 4.3 kb linear substrate. Protein (1 pmol) was incubated with 100 µg/ml Etoposide (lanes 2–3) or 50 µg/ml mAMSA (lanes 5–6) at 37°C for 1 h before addition of 0.1% SDS and 0.5 mg/ml proteinase K. Reaction products were run overnight on a 1% agarose gel. Lanes 1 and 4 are with no protein, lanes 2 and 5 are with WTβ, and lanes 3 and 6 are βG465D.