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. 2006 Mar 20;34(5):1620–1632. doi: 10.1093/nar/gkl060

Figure 2.

Figure 2

Kinetics of wild-type and S326C OGG1. Wild-type and S326C OGG1 (2.5 nM) were incubated with increasing amounts (3.25–100 nM) of duplex 8-oxoguanine substrates having C (A), T (B), G (C) or A (D) opposite 8-oxoG, or with a substrate having an abasic site opposite C (E). Glycosylase reactions with 8-oxoguanine paired with C, T and G, were incubated for 15 min at 37°C. AP-lyase reactions with the AP·C substrate and glycosylase reactions with 8-oxoG opposite A were incubated for 1 h at 37°C. Reactions were terminated and analyzed as described in Materials and Methods. Data points are means of three independent experiments with standard deviation.