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. 2006 Mar 20;34(5):1620–1632. doi: 10.1093/nar/gkl060

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Size-exclusion chromatographic analysis of wild-type and S326C OGG1. (A) Non-denatured protein size markers (Sigma). Peak 1, BSA dimer (132 kDa); peak 2, BSA monomer (66 kDa) and peak 3, carbonic anydrase (29 kDa). Purified wild-type OGG1 (100 µg) was analyzed on a Superdex 200 HR column equilibrated with 20 mM Tris–HCl (pH 7.4), 300 mM NaCl at a flow rate of 0.25 ml/min (B). Identical runs were performed with 100 µg polymorphic S326C OGG1 (C) or 100 µg of both wild-type and S326C OGG1 together (D).