Table 1.

PCR primers used for amplification and mutagenesis with underlined restriction sites and bold typed mutated codons

Amplification primers
B.subtilis ssb forward	GCGGATCCATGCTTAACCGAGTTGTATTAGTCGG	BamHI
B.subtilis ssb reverse	AAAACTGCAGTTAGAATGGAAGATCATCATCCGAG	PstI
B.subtilis ywpH forward	GCGGATCCATGTTCAATCAGGTCATGCTTGTCGGACG	BamHI
B.subtilis ywpH reverse	AAAACTGCAGTTAATCAGCAGCTTTTTCCCGGGGTTTAGGG	PstI
B.subtilis ssb SP forward	GCGAATTCTTCCGAAGTTTATTCTTGACATGGGATCAACTTCGCGTATAATAG	EcoRI
	GTAATTGTGAGTAATAGAATTATTGCTCCTTGCCCATTATGGGCCGCTTAGTCC
	AAAAGGAGGTGCAAACAGATGCTTAACCGAGTTGTATTAGTCGGAAGAC
B.subtilis ssb HT reverse	GCGGATCCTTATCCGTGATGGTGATGGTGATGGAATGGAAGATCATCATCCGA	BamHI
	GATGTCAATCG
E.coli ssb forward	CGGGATCCATGGCCAGCAGAGGCGTAAACAAGG	BamHI
E.coli ssb reverse	TTTTCTGCAGTCAGAACGGAATGTCATCATCAAAGTC	PstI
S.coelicolor ssbL forward	CGGGATCCATGGCAGGCGAGACCGTCATCACGGTC	BamHI
S.coelicolor ssbL reverse	TTTTCTGCAGTCAGAAGGGGGGCTCGTCCGAGTAGCCGCC	PstI
Mutagenic primers
B.subtilis ssb Y82F forward	CAAACAAGAAACTTTGAAAACCAGCAAGGACAGCGTGTCTTCGTG	NA
B.subtilis ssb Y82F reverse	GCTGGTTTTCAAAGTTTCTTGTTTGTAAACGGCCATCTACGCC	NA
Primers for gene inactivation with pMUTIN-2
B.subtilis ywqD forward	CGGGATCCGGCAAATGAAATCAGTCATGATTACATCGGC	BamHI
B.subtilis ywqD reverse	ATAAGAATGCGGCCGCTAACCAGAACAGAACCGTCTGCCACGTTGCC	NotI