FIG. 7.

Transferrin receptor recycling is unaffected by the lack of PKCβII activity. HeLa cells were transiently transfected with the PKCβII wild-type or T500V plasmid for 16 h. Alexa 594-labeled transferrin (50 μg/ml) was then bound to serum-starved cells for 20 min at 4°C. Cells were then shifted to 37°C for 15 min, washed in low-pH glycine to remove any uninternalized ligand, and returned to 37°C for 15 min to monitor receptor recycling. Cells were analyzed by fluorescence microscopy with an antibody to transferrin receptor. Bars = 8 μm.