FIG. 1.

Comparison of salt tolerance exhibited by the DHAL2 gene and S. cerevisiae HAL2 (ScHAL2). (A) Growth curve of strain RS16 carrying plasmid pRS425, pMA29 (ScHAL2), or pMA7 (DHAL2). Cultures were grown to early log phase (A₆₀₀ of ∼0.5) and reinoculated at A₆₀₀ of 0.05 in leucine minimal medium with or without 0.2 M LiCl. Growth of the cultures was followed by their absorbance at 600 nm. (B) Growth curve of strain RS1051 carrying plasmid pMA27 (DHAL2) or pMA30 (ScHAL2) in a low-copy-number vector. Cultures were grown to early log phase (A₆₀₀ of ∼0.5) and reinoculated at A₆₀₀ of 0.05 in methionine minimal medium with or without 0.2 M LiCl. Growth of the cultures was followed by their absorbance at 600 nm. The experiment was repeated three times with similar results.