FIG. 6.

Analysis of DHAL2 expression in D. hansenii. Immunoblots showing localization of Dhal2p. Membrane (P) and cytosolic (S) fractions from D. hansenii strain MTCC234 grown with or without NaCl (A), LiCl (B), KCl (C) and sorbitol (D) at indicated concentrations were made as described in Materials and Methods. A total of 20 μg of protein from each fraction was subjected to SDS-PAGE followed by immunoblotting with anti-Dhal2p antibody. The experiment was repeated five times with similar results.