FIG. 8.
Growth pattern of S. cerevisiae expressing Dhal2p under high salt stress conditions. Cultures of strain RS1051 expressing the full-length DHAL2 ORF (pMA27) or the truncated DHAL2 ORF (pMA28) under the regulation of HAL2 promoter was grown to early log phase (A600 of ∼0.5) and reinoculated at A600 of 0.05. Growth of the cultures was followed by measuring their absorbance at 600 nm. (A) Growth curve in methionine minimal medium. (B) Growth curve in methionine minimal medium containing 0.4 M LiCl. The experiment was repeated three times with similar results.