TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| RS16 | MATaura3-251, 328, 372 leu2-3,112 | 17 |
| RS1051 | MATaura3-251, 328, 372 leu2-3,112 hal2::URA3 | 19 |
| MTCC234 | D. hansenii | MTCC, India |
| Plasmids | ||
| pRS425 | LEU2 2μ | 8 |
| p414TEF | TRP1 CEN ARS1 | 22 |
| pRS-GFP | URA3 CEN ARS1 | 16 |
| pMA7 | LEU2 2μ DHAL2 | 1 |
| pMA14 | TRP1 CEN ARS1 DHAL2 (full-length) | 1 |
| pMA15 | TRP1 CEN ARS1 DHAL2 (truncated) | 1 |
| pMA23 | TRP1 CEN ARS1 DHAL2 (M3 ATG mutant) | This study |
| pMA24 | TRP1 CEN ARS1 DHAL2 (M1/M2 ATG mutant) | |
| pMA25 | URA3 CEN ARS1 DHAL2-GFP (M3 ATG mutant) | |
| pMA26 | URA3 CEN ARS1 DHAL2-GFP (M1/M2 ATG mutant) | |
| pMA27 | TRP1 CEN ARS1 DHAL2 (full-length; under HAL2 promoter) | |
| pMA28 | TRP1 CEN ARS1 DHAL2 (truncated; under HAL2 promoter) | |
| pMA29 | LEU2 2μ HAL2 | |
| pMA30 | TRP1 CEN ARS1 HAL2 (under HAL2 promoter) |