FIG. 6.

Effects of HA-Myb1 overexpression on ap65-1 transcription in T. vaginalis. T. vaginalis T1 cells (lanes 1 and 2) or pFLPha-myb1/TUBneo-transfected cells (lanes 3 and 4) were grown under iron-replete (lanes 1 and 3) or iron-depleted (lanes 2 and 4) conditions for 8 h (left panel) and 18 h (right panel). Expression levels of individual mRNA species were analyzed by RT-PCR. Using 1 microliter of 2×-diluted first-strand cDNA as a template, the ap65-1 signals at 8 h and 18 h were detected after 23 cycles and 21 cycles, respectively. Using 1 μl of 4×-diluted first-strand cDNA as a template, the β-tubulin signal was detected after 26 cycles, and myb1 and ha-myb1 signals were detected after 28 cycles. The PCR products were assayed by agarose gel electrophoresis with ethidium bromide staining.