TABLE 1.
Sequences of oligonucleotides used for this study
| Primer name and purpose | Sequence (5′ to 3′) |
|---|---|
| RT-PCR | |
| ap65-1-5′ | AATGGCAAGGCCCTCTGCGCTAC |
| ap65-1-3′ | TAAATTAAGAAAGCTAAGTGTTTAAAAATCGCGC |
| tub-5′ | AAATCGTTCACATCCAAGCTGG |
| tub-3′ | TTGTATGGCTCGACGACTGTATCAG |
| ha-myb1-5′ | GATTACGCTCTTATGATGTTTG |
| myb1-5′ | TGCCTTTAATCGCATGATGTTTG |
| myb1-3′ | ATTATAATCAGTATCGACAGAGGCG |
| Southwestern screening | |
| mre-2sa | CAGAGCTGTATCGTCTAGCTGTATCGTCTAGCTGTATCGTCTAGC |
| mre-2as | GCTAGACGATACAGCTAGACGATACAGCTAGACGATACAGCT |
| Plasmid constructionb | |
| flp-5′-sac2 | TCCGCGGCTACTATAGGGCACGCGTGGTC |
| flp-3′-nco1 | ACCATGGATCCCATCATAAAAGTGATATGAA |
| flp-3′-bamh1-ha-hind3 | AGGATCCAAGAGCGTAATCTGGAACATCGTATGGGTAGGAAAGCTTCTCTTCTGTCGCC |
| ha-myb1-5′-bgl2 | CAGATCTTACCCATACGATGTTCCAGATTACGCTCTTATGATGTTTGACGGCCTTTCCG |
| myb1-5′-nde1 | TTCATATGATGTTTGACGGCCTTTCCG |
| myb1-3′-sal1 | TTGTCGACGGAAGCAACACTTAACTTAAAACG |
| ΔN(103)myb1-5′-nde1 | TTCATATGAAAGTCAAGTTCACGGAAGAGG |
| ChIP | |
| 1f | GGGCGAAATCACGGAAATCG |
| 1r | CCTTGCGGGAGAGTTCACGTGCTGG |
| 2f | TTCCAATCAACATTTATCTATACTATCGG |
| 2r | TGCCATTGCGATGATTATGGTTG |
| 3f | GCATTCAGTTTTGATCCAGGTTTCC |
| 3r | GATAGTATAGATAAATGTTGATTGGAAGC |
| 4f | GTTCTTATTCAGATGTTCCTGATTTG |
| 4r | GCCCTAGTATAGCATTCAAATGATCC |
a
The DNA sequence of MRE-2f is indicated in bold.
b
Sequences of restriction enzyme sites, as indicated in the names, are underlined.