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Deletion of AZF1 produces a growth defect on nonfermentable medium at 37°C. Wild-type (WT) (BY4741) and azf1Δ (TTL9) mutant strains were plated on the media indicated in serial 10-μl dilutions. Plates were incubated at 30°C or 37°C as indicated. YPEtOH, YEP-ethanol; YPG/L, YEP-glycerol-lactate.
