FIG. 2.

Azf1 regulates distinct gene sets depending on carbon source. Azf1-dependent genes were determined as described in Materials and Methods and are grouped by functional categories based on gene ontology information at SGD (http://www.yeastgenome.org/). Each Azf1-dependent group was examined for enrichment of promoter sequences by use of Regulatory Sequence Analysis Tools (RSAT, http://rsat.scmbb.ulb.ac.be/rsat/). AAAAGAAA was the most significant enriched sequence in both the glucose and the glycerol-lactate (Gly/Lac) gene set, with significance indexes of 3.72 and 2.88, respectively. The significance index is a −log₁₀ conversion of the expected number of patterns that would be returned at random for the motif's P value. Each gene is accompanied by a representation of its promoter (up to 800 bp upstream, with each tick marking 100 bp). Rectangles represent the positions of A₄GA₃ elements. ER, endoplasmic reticulum.