FIG. 3.

Deletion of AZF1 produces cell wall defects. (A) Wild-type (WT) (BY4741) and azf1Δ (TTL9) mutants were serially diluted and plated in 5-μl drops on YEP-glycerol-lactate media with the indicated cell wall disruptors as described in Materials and Methods. Plates were incubated for 2 days at 30°C. CFW, calcofluor white. (B) WT (BY4741) and azf1Δ (TTL9) mutants were grown to mid-log phase in either YEP-glycerol-lactate (GL) or YEPD (D) medium. Aliquots were resuspended in zymolyase (0.05 U/μl) as described in Materials and Methods, and cell disruption by zymolyase was measured by monitoring the OD₆₀₀ at the indicated intervals. Error bars represent the standard deviations of this ratio across triplicate experiments.