FIG. 10.
Induction of GCN4 translation in yeast cells expressing an inactive Na,K-ATPase (A) or the rat PACR1 receptor (B) or overexpressing the endogenous PMA1 gene (B). Yeast cells carrying the translational GCN4-lacZ reporter fusion and an expression plasmid for α1(D369A)β1-Na,K-ATPase, rat PACR1, or yeast PMA1 were grown at 30°C as described in Materials and Methods. Protein production was induced at an OD450 of 1.0 with 2% galactose dissolved in medium lacking glucose. β-Galactosidase activities were determined in purified cytosolic fractions. The results shown are from two or three independent growth experiments with triple determinations of each data point.