FIG. 14.

Measurement of cytoplasmic (A) and vacuolar (B) amino acid pools in the Na,K-ATPase-expressing strain and the control strain carrying the empty vector. Results from the Na,K-ATPase-expressing cells and the corresponding control cells are grouped. Cells were grown at 30°C in amino acid-supplemented minimal medium with 0.5% glucose and 3% glycerol until the OD₄₅₀ reached 1.0 and then induced with 2% galactose dissolved in growth medium. The amino acid pools were determined by the ninhydrin method, as described in Materials and Methods. The results shown are from two independent experiments.