FIG. 3.

Effect of heterologous expression of Na,K-ATPase on polysome distribution. S288C mutant yeast cells expressing either Na,K-ATPase or no heterologous protein were grown as described in Materials and Methods. At an OD₄₅₀ of 0.3, each culture was induced with 2% galactose dissolved in growth medium lacking glucose but including amino acids. Polysomes were prepared from cells harvested at the indicated time points and analyzed as described in Materials and Methods.